Easyselect pichia expression kit manual

 · pastoris X and BMY(G) medium used for protein expression were prepared according to the EasySelect™ Pichia Expression Kit manual. For the preparation of transformed P. pastoris cells. Invitrogen, () EasySelect ™ Pichia Expression Kit A Manual of Methods for Expressi on of Recombinant Proteins Using pPI CZ and pPICZ . according to protocols suggested by the EasySelectTM Pichia Expression Kit manual, Invitrogen (USA). Finally, it was transformed into M. guilliermondii strain SO. The cloning process was completed according to FLDα manual (Invitrogen ). The transformant mixture was spread onto YPDS (w/v) (1% yeast extract, 2% peptone, 2%.

Original Pichia Expression Kit: Contains the strains, GS and KM71, plus 4 vectors and reagents. Geared for histidine selection. K EasySelect Pichia Expression Kit: Contains the strains GS, X and KM71, along with the pPICZ vectors and reagents. K Multi-Copy Pichia Expression Kit. The treatments described below were applied on cells grown in BMGH medium (pH ; Invitrogen EasySelect™ Pichia Expression Kit manual) supplemented with 1/50 (v/v) of a solution containing mM MnSO 4 and 50 mM MgSO 4. The cell pellets from centrifuged inocula were suspended in BMGH medium. Schematic (adapted from the Invitrogen EasySelect Pichia expression kit manual) of the integration of the pPICZB vector into the P. pastoris genome by gene insertion. Double headed arrows indicate the sequence that was amplified using the PpAOX1 TT primer pair when quantifying incorporated plasmids by qPCR.

EasySelectŽ Pichia Expression Kit Version G EasySelectŽ Pichia Expression Kit A Manual of Methods for Expression of Recombinant Proteins Using pPICZ and pPICZα in Pichia pastoris Catalog no. K www.doorway.ru tech_service@www.doorway.ru EasySelect ™ Echo -adapted Pichia Expression Kit pPICZ-E Echo™-adapted Expression Vector Kit pPICZα-E Echo™-adapted Expression Vector Kit EasySelect™ Pichia Strains Kit Pichia EasyComp™ Kit Pichia Media Kit ET pUni/V5-His-TOPO® TA Cloning Kit ETC pUniBlunt/V5-His-TOPO® Cloning Kit ETC pUni/V5-His A, B and C ETC. The pPICZ A, B, and C expression vectors may be used with the Original Pichia Expression Kit, and are included in the EasySelect™ Pichia Expression Kit (see page v for ordering information). Additional general information about recombinant protein expression in Pichia pastoris is provided in the manuals for.