Chelating sepharose manual

Chelating Sepharose Fast Flow is supplied pre-swollen in 20% ethanol. Prepare a slurry by decanting the 20% ethanol solu-tion and replacing it with distilled water in a ratio of 75% sett-led gel to 25% distilled water. 3 I Introduction Immobilized metal ion affinity chromatography (IMAC) exploits a molecule’s affinity for chelated metal ions. TheFile Size: 51KB. Ni Sepharose excel and HisTrap excel (columns prepacked with Ni Sepharose excel) are products for IMAC protein purification. Ni Sepharose excel consists of 90 μm highly cross-linked agarose beads, to which a chelating ligand has been coupled. The ligand is precharged with exceptionally strongly bound nickel ions. The nickel ions remain bound even. This chelating group has then been charged with nickel (Ni2+) ions. Ni Sepharose 6 Fast Flow has low Ni2+ leakage, high protein-binding capacity, and is compatible with a wide range of additives used in protein purification. Its high flow properties make it excellent for scale-up. Ni Sepharose 6 Fast Flow is available in 5, 25, , ml.

Chelating Sepharose Fast Flow consists of iminodiacetic acid groups coupled to Sepharose Fast Flow by stable ether linkages via a 7-atom spacer. Chapter IV contains specific methods for purification of histidi-ne-tagged fusion proteins using gravity columns or centrifu-gaion. Chelating Sepharose Fast Flow belongs to the BioProcess™ Media family. TALON® Metal Affinity Resins User Manual PT www.doorway.ru Clontech Laboratories, Inc. A Takara Bio Company Page 3 of 15 B. Overview of TALON Resin Formats TALON Metal Affinity Resin is useful for batch and low-pressure chromatographic applications. This resin utilizes Sepharose CL-6B (GE Healthcare), a durable substrate that performs very well. 5 10 15 20 25 ml 0 A nm Column: HiTrap Chelating HP, 1 ml, Cu2+-loaded according to the instructions Sample: µl egg white (10% in binding buffer filtered through a glass filter) Metal ion: Cu2+ Binding buffer: M sodium phosphate, 1 M NaCl, pH Elution buffer: M sodium phosphate, 1 M NH 4Cl, pH Flow rate: ml/min Gradient: 8 ml linear gradient 0% to % in.

Chelating Sepharose Fast Flow consists of iminodiacetic acid groups coupled to Sepharose 6 Fast Flow by stable ether linkages and sufficiently long spacer arms (Fig 2). The Sepharose 6 Fast Flow base matrix is a highly cross-linked, 6% agarose with excellent chemical and physical stability. The cross-linking of the matrix has been optimized to give. of histidine-tagged proteins. The IMAC medium Ni Sepharose™ High Performance consists of 34 µm beads of highly cross-linked agarose, to which a chelating group has been coupled. This chelating group has then been charged with nickel (Ni2+) ions. Ni Sepharose High Performance has low Ni2+ leakage, high protein-binding capacity. Chelating Sepharose Fast Flow, 50 mL. Chelating Sepharose Fast Flow is an uncharged IMAC chromatography resin for purification of proteins and peptides with affinity for metal ions. Ready to charge with your metal ion of choice for optimized selectivity.